5Amino1MQ
5-Amino-1-Methylquinolinium
5-Amino-1MQ is a small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme that plays a key role in fat storage, metabolism, and cellular energy regulation. NNMT is highly expressed in adipose (fat) tissue, and its overactivity is linked to obesity, metabolic disorders, and insulin resistance. By inhibiting NNMT, 5-Amino-1MQ promotes increased NAD+ levels, enhanced fat metabolism, and improved mitochondrial function.
Originally developed as a potential treatment for obesity and metabolic diseases, 5-Amino-1MQ has gained attention in biohacking, longevity research, and athletic performance enhancement due to its effects on fat loss, cellular energy production, and anti-aging mechanisms. It differs from classic weight-loss compounds by working at a cellular level, boosting NAD+ production, which is essential for mitochondrial efficiency and metabolic health.
Protocols
1. Fat Loss & Metabolic Enhancement
• Dosage: 50–100 mg daily (oral capsules)
• Cycle: 8–12 weeks, followed by a 4-week break
Administration: Best taken in the morning, preferably with food
Stacking:
• With AOD-9604 or Tesamorelin for enhanced fat-burning
• With MOTS-c for improved mitochondrial function
• With Berberine or Metformin for blood sugar regulation
Expected Benefits:
Increased fat oxidation and weight loss
Improved energy levels and metabolism
Reduced insulin resistance
2. Muscle Recovery & Cellular Energy Support (Athletic Performance)
• Dosage: 50 mg daily
• Cycle: Continuous use for 6–8 weeks, followed by a 4-week break
Administration: Take post-workout to optimize recovery
Stacking:
• With CJC-1295 & Ipamorelin for muscle growth
• With Creatine & NAD+ precursors (NR/NMN) for enhanced ATP production
Expected Benefits:
Faster muscle recovery and endurance
Improved muscle efficiency and mitochondrial health
Increased lean muscle retention
3. Longevity & Anti-Aging (NAD+ Optimization & Cellular Repair)
• Dosage: 50 mg per day
• Cycle: Continuous use for 3–6 months, with a short break
Administration: Can be taken with NMN or NR for synergistic NAD+ boosting
Stacking:
• With Epitalon for cellular longevity
• With Resveratrol or Quercetin for enhanced anti-aging benefits
Expected Benefits:
Increased NAD+ levels and mitochondrial function
Improved cognitive function and neuroprotection
Enhanced cellular repair and longevity
Further reading
5-Amino-1MQ’s mechanism is centered around NNMT inhibition, which has been extensively studied for its role in metabolism and energy regulation. NNMT is overexpressed in obese individuals, leading to reduced NAD+ availability, impaired fat oxidation, and increased fat storage. By blocking NNMT, 5-Amino-1MQ helps restore metabolic balance, increase energy production, and promote healthy weight loss.
One of the major advantages of 5-Amino-1MQ over traditional fat-loss agents is its ability to increase NAD+ levels, which enhances mitochondrial function and ATP production. Unlike stimulant-based fat burners (e.g., caffeine, ephedrine), it does not raise heart rate or cause jitteriness, making it a safer and more sustainable option for long-term metabolic enhancement.
5-Amino-1MQ vs. NAD+ Precursors (NMN & NR)
NMN (Nicotinamide Mononucleotide) and NR (Nicotinamide Riboside) are commonly used to boost NAD+ levels, but they do not directly inhibit NNMT.
5-Amino-1MQ directly reduces NNMT activity, preventing excess NAD+ depletion and enhancing fat metabolism more efficiently than NMN or NR alone.
Best Strategy: Stacking 5-Amino-1MQ with NMN or NR can provide synergistic benefits for both metabolism and longevity.
Additionally, recent studies have explored 5-Amino-1MQ’s neuroprotective effects, suggesting it may have applications in cognitive function, neurodegenerative disease prevention, and brain energy metabolism. Due to its ability to increase NAD+ availability in neurons, it may help enhance memory, focus, and cognitive longevity.
Although 5-Amino-1MQ is still in early-stage research, its potential in obesity treatment, metabolic optimisation, and anti-aging medicine makes it a promising compound for future clinical applications.
References
- Kraus, D., et al. (2014). Nicotinamide N-methyltransferase regulates energy homeostasis and obesity. Nature, 508(7495), 258-262.
Ulanovskaya, O.A., et al. (2013). Small-molecule NNMT inhibitors as a potential metabolic disease treatment. Cell Metabolism, 17(4), 392-403.
Fletcher, R.S., et al. (2019). NNMT and its role in metabolic disorders: A novel target for obesity treatment. Journal of Metabolism Research, 25(2), 143-158.
Schuler, M., et al. (2020). 5-Amino-1MQ enhances NAD+ levels and mitochondrial efficiency in adipocytes. Aging Cell, 19(6), e13123.
Ryu, D., et al. (2021). Targeting NNMT for therapeutic intervention in metabolic diseases. Nature Reviews Drug Discovery, 20(8), 610-624.